The age-related functional changes in skin are accompanied by morphological and biochemical changes. However, since spatial information has been lost after isolation and culturing of cells from tissue, it remains largely unknown how diverse cell populations and their microenvironment are spatially coordinated in a three-dimensional (3D) skin architecture and how its impairment can cause age-dependent skin dysfunction. Our work will provide an imaging-based platform that can quantitatively measure morphological and molecular changes of aging skin at single cell level. We will develop induced pluripotent stem cells (iPSC)-derived skin organoids and mouse models of premature aging and evaluate their phenotypes by multiplex imaging.